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KMID : 0602720080120010004
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Implantology
2008 Volume.12 No. 1 p.4 ~ p.11
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Effect of dental Implant micro thread dimensions on osteoblasts
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Koo Ki-Tae
Kim Young-Jin
Kim Byoung-Kook
Kang Eun-Jung
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Abstract
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The present study was undertaken to evaluate the effect of micro thread dimensions on adehesion, proliferation and differentiation of osteoblasts on titanium surface. Scanning electron micrographs of the micro thread titanium surfaces and machined surface (control) confirmed osteoblast attachment and proliferation. Cells showed a flattened and fibroblast-like morphology. Osteoblast adhesion was estimated by measuring the number of metabolically viable cells present after removing the unattached cells with PBS buffer (PH 7.2) after 1 hour. There were statistically significant differences between G2 (pitch 0.2/depth 0.1) and the control group while groups G2 (pitch 0.2/depth 0.1) and G3 (pitch 0.3/depth 0.15) had smaller differences between them still reaching statistical significance. Groups G1 and G2 showed better osteoblast attachment than the control group. However, this difference did not reach statistical significance. Cell proliferation was measured using the MTS assay at days 1, 3, 5 post culturing of the 4 groups. All three test groups showed statistically significant difference in cell proliferation when compared to the control group. This was true for all three days and the difference among the three groups was marginal not reaching statistical significance. Greater cell proliferation was observed for group 2, group 3, group 1 and control, successively. Cell differentiation was measured on days 4, 7, and 14 using the ALP assay. On days 4 and 14, results show that only slight differences exist between test and control groups. This was also true between the three test groups. On day 7 however, ALP activity of the G2 group was higher than the other groups reaching statistical significance. ALP activity between G1, G3 and the control group did not reach statistical significance. Within the limitations of the study, it may be concluded that titanium discs with micro thread structures provided better osteoblast adhesion and proliferation compared to the control without the micro thread structures. Also, it may be suggested that micro thread dimension of pitch may be one way to optimize implant stability in suboptimal clinical conditions.
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KEYWORD
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micro thread dimensions, osteoblast, adhesion, proliferation, differentiation
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